RT期刊文章SR电子T1血清学诊断动摩根富林明神经学神经学乔FD Lippincott Williams &威尔金斯SP 665 O半岛投注体育官网P 671 10.1212 / WNL。0 b013e318248dec1 VO 78是9 A1 P.J.水域A1。麦肯A1 M.I.雷特A1 s Rajasekharan A1退役军人列侬A1。维拉波斯A1 j .宫A1 J.N. Mandrekar A1。文森特A1。酒吧或者A1中华民国Pittock年2012 UL http://n.neurolog半岛投注体育官网y.org/content/78/9/665.abstract AB目的:Neuromyelitis视(动)免疫球蛋白G(免疫球蛋白)(aquaporin-4 AQP4免疫球蛋白)是高度特定的动及相关疾病,和自身抗体检测已成为一个重要的调查患者的脱髓鞘疾病。然而,尽管不同的技术现在使用,没有进行多中心的比较。本研究比较了不同检测的敏感性和特异性,包括内部流仪测定和2商业化验(ELISA和转染细胞试验(CBA))。方法:6个试验方法(内部或商业)进行国际中心2使用编码从动(35例)患者血清,动谱系障碍(25例),复发缓和多发性硬化(39例),其他自身免疫性疾病(25例)和健康受试者(22科目)。结果:最高的敏感性被化验检测了免疫球蛋白结合细胞表达重组AQP4与定量流式细胞术(77;46 60)或视觉观察(CBA, 73%; 44 of 60). The fluorescence immunoprecipitation assay and tissue-based immunofluorescence assay were least sensitive (48%–53%). The CBA and ELISA commercial assays (100% specific) yielded sensitivities of 68% (41 of 60) and 60% (36 of 60), respectively, and sensitivity of 72% (43 of 60) when used in combination. Conclusions: The greater sensitivity and excellent specificity of second-generation recombinant antigen-based assays for detection of NMO-IgG in a clinical setting should enable earlier diagnosis of NMO spectrum disorders and prompt initiation of disease-appropriate therapies. AQP4=aquaporin-4; CBA=cell-based assay; E=EUROIMMUN; FACS=fluorescence-activated cell sorting; FIPA =fluorescence immunoprecipitation assay; IgG=immunoglobulin G; IIF=indirect immunofluorescence; M=Mayo; MS=multiple sclerosis; NMO=neuromyelitis optica; NMOSD=neuromyelitis optica spectrum disorder; O=Oxford; R=RSR/Kronus; ROC=receiver operating characteristic curve