作者@article {Waters665 = {P.J.水域和a·麦肯M.I.雷特和美国Rajasekharan退役军人列侬和维拉波斯a . j .宫殿和J.N. Mandrekar和a·文森特·a .酒吧或中华民国Pittock}, title ={血清学诊断动},体积={78}={9},页面= {665 - 671}= {2012},doi = {10.1212 / WNL。出版商0 b013e318248dec1} = {Wolters Kluwer健康,公司代表美国神经病学学会},文摘={目的:Neuromyelitis视(动)免疫球蛋白G(免疫球蛋白)(aqu半岛投注体育官网aporin-4 AQP4免疫球蛋白)是高度特定的动及相关疾病,和自身抗体检测已成为一个重要的调查患者的脱髓鞘疾病。然而,尽管不同的技术现在使用,没有进行多中心的比较。本研究比较了不同检测的敏感性和特异性,包括内部流仪测定和2商业化验(ELISA和转染细胞试验(CBA))。方法:6个试验方法(内部或商业)进行国际中心2使用编码从动(35例)患者血清,动谱系障碍(25例),复发缓和多发性硬化(39例),其他自身免疫性疾病(25例)和健康受试者(22科目)。结果:最高的敏感性被化验检测了免疫球蛋白结合细胞表达重组AQP4与定量流式细胞术(77;46 60)或视觉观察(CBA 73 \ %;44 60)。荧光免疫沉淀反应试验和组织免疫荧光试验至少敏感(48 \ % {\ textendash} 53 \ %)。 The CBA and ELISA commercial assays (100\% specific) yielded sensitivities of 68\% (41 of 60) and 60\% (36 of 60), respectively, and sensitivity of 72\% (43 of 60) when used in combination. Conclusions: The greater sensitivity and excellent specificity of second-generation recombinant antigen-based assays for detection of NMO-IgG in a clinical setting should enable earlier diagnosis of NMO spectrum disorders and prompt initiation of disease-appropriate therapies. AQP4=aquaporin-4; CBA=cell-based assay; E=EUROIMMUN; FACS=fluorescence-activated cell sorting; FIPA =fluorescence immunoprecipitation assay; IgG=immunoglobulin G; IIF=indirect immunofluorescence; M=Mayo; MS=multiple sclerosis; NMO=neuromyelitis optica; NMOSD=neuromyelitis optica spectrum disorder; O=Oxford; R=RSR/Kronus; ROC=receiver operating characteristic curve}, issn = {0028-3878}, URL = {//www.ebmtp.com/content/78/9/665}, eprint = {//www.ebmtp.com/content/78/9/665.full.pdf}, journal = {Neurology} }